FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4101860
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18300.035.0142568
GTCTCGC18550.031.8597281
CTGTCGC23500.030.0236999
TCGCTCT20850.029.6960284
GTCTTGC40900.028.8996541
GGTATAC8550.024.4457841
TCTCGCT23900.024.1248262
GTCTCAC51000.023.6003481
GCGTATA2202.335245E-522.9322931
GTATTAG15400.022.9322911
CGCTCTG23550.022.9285355
GTCTTAC18000.022.4226861
GTGTAAA23350.021.6064411
GTGTAAC13050.021.5390321
CTCGCTC24800.021.5056293
TACACTG28300.021.369725
GTGTAGA23450.021.2069571
GTATAAC8000.020.7209631
GTATAGG11850.020.679151
GTATAAG11350.020.3201181
Produced by FastQC (version 0.11.5)