FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389342
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13700.038.362368
GTCTCGC15800.038.322241
CTGTCGC17950.033.290229
GTCTTGC32100.032.111431
TCTCGCT18750.030.370682
TCGCTCT17050.027.8690744
CTCGCTC19500.026.6150073
GTGTAAC11000.025.5564541
GTATAGA10450.025.5219631
GCGATAC1450.001269076624.8559673
GTACTAC5950.024.2293471
GTATTAC8650.024.1663231
GTATTAT10150.024.1457981
GTCTCAC41300.023.2129461
CGCTCTG20450.022.5314775
GTGTTAC14800.022.4039611
TTGCTCT43000.022.1008244
GTACTAT7250.021.8732531
GTGTAAG17150.021.855861
GGAGTGC83550.021.6286935
Produced by FastQC (version 0.11.5)