FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3389342
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1459.196083E-734.7561075
TCTGTCG15000.034.556998
GTCTCGC14350.031.6438851
TCGCTCT14300.031.2145274
CTGTCGC18500.028.406669
GTCTTGC31550.028.3284931
GTGTAAC10000.025.9479851
GTCTCAC41150.024.8725131
GTATTAC7450.024.1871591
TTGCTCT44050.023.5351894
CTCGCTC17550.023.3829233
TACACCG3153.0910087E-822.855495
CGCTCTG17400.022.7569755
GTATTAG11450.022.6619951
GTACTAC6550.022.008471
GAGTGCA83450.021.9996056
TCTCGCT18350.021.9912782
GGTATAC6900.021.9367031
GTGTTAC15250.021.741481
GTATAGG9200.021.1532481
Produced by FastQC (version 0.11.5)