FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359140
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13100.040.1192978
GTCTCGC14950.035.6760981
CTGTCGC16950.032.2809039
GTCTTGC31950.031.8079071
TCGCTCT16750.030.087424
TCTCGCT19550.026.9130522
GTATTAG11100.025.3237761
GTCTCAC38850.024.674451
GTGTTAG26150.023.703561
GTATTAC7700.023.4010941
TTGCTCT45900.023.3708954
CTCGCTC20150.023.2501183
GTCTTAC12500.023.0641161
TTATACT9450.022.8555244
CGCTCTG21050.022.5732235
GCGCTAG3554.4237822E-922.3332121
TACACTG20950.021.3063665
GTTTCAC52850.021.2748473
GGCTAGA21400.021.2184491
GTACTAA6800.021.1986371
Produced by FastQC (version 0.11.5)