FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359140
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC33750.034.6117441
TCTGTCG15850.033.1586238
GTCTCGC16600.032.144441
TCGCTCT17250.031.7195744
CTGTCGC18500.028.795899
GTACGAC1306.7566585E-427.6904183
TCTCGCT19300.026.8922982
ACCGTGT10500.026.7410328
CGCTCTG18600.026.3207865
CTCGCTC19800.026.1800333
GTGTAAA18300.025.2180271
GTATAAG8600.025.1538831
GTATTAG11050.024.797251
GTATAAC7050.023.524531
GTACTAT7750.023.260581
GGGTTTC54100.023.1917881
GTTTCAC48900.023.1149863
GTATTAC7500.023.0744931
TTGCTCT45500.022.627034
GCGTTAC2252.7675125E-522.4335351
Produced by FastQC (version 0.11.5)