FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2810763
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12050.039.432458
GTCTCGC13450.038.0496671
TCGCTCT14150.033.071494
GTCTTGC27500.032.501531
CTGTCGC15800.030.0734839
TCTCGCT16050.029.6403752
GTATTAC6650.029.2655661
CTCGCTC16750.027.5410143
CGCTCTG16650.026.808615
GTGTTAG20900.025.176271
GGCTAGA17500.024.7131441
TTGCTCT37950.024.4723014
TAATACT8650.024.1367454
GTCTCAC30800.023.6366231
CTTGCTC37050.022.9566543
GTATTCG1902.2825907E-422.7621061
TAATACG2003.2554584E-421.5982284
GTGTAAG15150.021.4099031
GTGTAGG16900.021.3254431
GTATTAG9950.021.0082441
Produced by FastQC (version 0.11.5)