FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961980
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.040.19958
TCGCTCT15250.033.048774
CTGTCGC15500.032.5157249
GTCTCGC14600.031.5788441
GTATTAC6700.030.1059471
GTCTTGC26950.029.1364651
CGTTTCG1255.369757E-428.799649
TCTCGCT16600.028.2081362
GTATACG1804.9432456E-628.0152591
CTCGCTC17600.027.8333423
GTATTAA8600.027.6429631
CGCTCTG17000.026.682025
GTCTCAC35400.026.0480841
GGTATAC6150.025.7701341
TCCGTCG1400.001041199525.7139668
GTGTTAC12000.023.4127521
GTATAAA12150.023.1237051
GTATTAG10000.023.0525551
GTGTAGA16050.022.890971
GTTTCAC47500.022.7492333
Produced by FastQC (version 0.11.5)