FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357636
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12200.036.6068881
TCGCTCT13350.035.055844
TCTGTCG10550.033.4404348
GTCTTGC20200.033.1636661
TCTCGCT14450.030.4083582
GTATTAT6150.028.1104031
CTCGCTC15200.027.4862423
CTGTCGC13400.027.4027189
CGCTCTG15000.026.879745
CGTTACC1658.801859E-526.1937852
GTCTTAC10250.025.2993621
GTACACG2601.220069E-724.9344671
GTGTAAG11100.024.6599141
TAATACT7100.024.3377914
GTGTTAG17300.024.1497611
GTATTAG7500.024.0109711
GTCTCAC28300.023.9261251
GGTATAC4950.023.2833651
GTATATA9350.023.1121641
TCTGTCA29800.022.711198
Produced by FastQC (version 0.11.5)