FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357636
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC23900.042.477611
GTCTCGC10200.036.0005261
TCTGTCG10600.035.3205268
TCGCTCT11250.031.9997924
GTATAAT5700.030.3162361
CTCGCTC12050.029.8753263
CTTGCTC30400.027.7103483
GTCTCAC25400.027.496471
GTATTAG6700.026.8660661
TTGCTCT31300.025.993444
TCTCGCT13300.025.9853462
GTGTTAG14850.025.697351
CTGTCGC15150.025.66329
CGCTCTG14000.024.6855555
GTAATAC5000.024.4798413
GTACCGT1500.001556207324.0003551
GTCTTAC8700.024.0003551
GTGTAAG11100.024.0003531
CAGCGTA2701.765111E-723.9998449
CTGTTGC25500.023.7174939
Produced by FastQC (version 0.11.5)