FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2582608
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.043.4935578
GTCTTGC24200.036.3028761
TCGCTCT12900.034.0462574
GTCTCGC13200.033.2776341
CTGTCGC17500.032.5026179
GTATTAG8350.030.1840931
GTACGAC1255.3694105E-428.7997863
TTGCTCT34250.027.9589184
GTCCTAC8400.027.4326171
CTCGCTC15050.027.26893
TACACTA6050.024.991555
CTTGCTC34100.024.703633
TCTCGCT16700.024.5784762
GGGTTTC43950.024.5770031
GTGTTAG16750.024.5051081
GTTCCGG3551.6916601E-1024.3416181
GTGTAAC8050.024.152631
CGTTTCG1500.001556448623.9998239
GTCTCAC33250.023.8230631
GTTTCAC37800.023.809353
Produced by FastQC (version 0.11.5)