FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.046.4876068
GTCTCGC11750.044.1278271
GTCTTGC26100.036.972791
TCGCTCT12700.036.283014
CTGTCGC17150.032.745949
TCTCGCT14550.032.1712762
GAACCGT2559.640644E-1131.0584356
GTACTAC3151.8189894E-1229.720131
CGCTCTG14000.029.313925
CTCGCTC15250.028.3275153
TTGCTCT33950.027.5696094
GTCTCAC28700.025.3429621
GTGTAAT10150.024.832471
GTGTTAG15400.024.7840941
GTGTAAA16100.024.6011121
GGGTTTC40150.024.5727041
CTTGCTC32750.024.1829013
TCTTGCT36350.023.9717032
TTACACT11850.023.6959064
TAGTACT5200.023.5381664
Produced by FastQC (version 0.11.5)