FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313938
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.048.8413548
GTCTCGC9950.039.1035081
CTGTCGC12050.038.2400789
TCGCTCT10750.037.5064054
TAGCGCG800.002107706935.999455
GTATTAG7450.035.784131
GTCTTGC21400.034.3424641
TCTCGCT11600.033.5413672
CTCGCTC11650.033.397423
CGCTCTG13300.029.2326345
GTACTAC3850.026.2006651
GTCTCAC26700.025.636421
TTGCTCT29850.025.0850434
GTTAGCC12450.024.8853723
GGGTTAC7550.024.812551
GTGTTAC8500.024.5823921
TAATACT6000.023.9996344
GTGTTAG16600.023.4385491
GTATTAT6950.022.8077751
CTACGCT1902.299886E-422.7364944
Produced by FastQC (version 0.11.5)