FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2751712
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC24950.031.7461361
TCTGTCG12700.031.1808728
GTGTTAG16400.030.7342721
GTCTCGC10750.028.132581
GTATAAT7400.026.2724651
GTATTCG1400.001040598625.716431
TTCACCG8250.025.30895
GTATTAC5800.024.8296591
CTGTCGC16550.024.79749
GTTTCAC36600.024.3932613
GTCTCAC30250.024.279711
ACCGTGT10200.023.999828
TCGCTCT12050.023.9002384
GTATAAA10300.023.7689721
TTGCTCT33600.023.5712534
GGAGTGC63750.023.4915895
GTGTAAA16250.023.4850371
GTATTAG8400.023.1447871
GGGTTTC41750.022.938441
CTTGCTC32100.022.6540373
Produced by FastQC (version 0.11.5)