FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499581
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11150.043.2942541
TCTGTCG10550.042.9946338
CTGTCGC13050.038.0684139
TCGCTCT11350.037.426774
GTCTTGC22550.033.228991
TCTCGCT13250.033.169912
CTCGCTC14000.030.8783113
CGCTCTG13400.030.6264175
GTTTCAC36500.029.609343
GGGTTTC39100.028.9303191
GTCCTAT6150.026.9453011
CGGTACT1658.830336E-526.1814374
TTGCTCT32350.025.594684
GGTTTCA39150.025.3967232
GTGTTAG17800.024.6910841
TTACGGT1751.3170321E-424.6853544
TCGAACT15600.024.4611829
GTGTAAC7150.024.1844141
GTATAAT7750.024.171411
GTACGTA1500.001550165124.0164661
Produced by FastQC (version 0.11.5)