FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499581
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11200.051.428428
GTCTCGC12100.041.6586041
TCGCTCT12950.041.142744
GTCTTGC23150.036.704821
CGCTCTG13450.036.401385
CTCGCTC15200.033.6314853
CTGTCGC17500.032.502769
TCTCGCT15400.031.7965682
TACCCCG2502.6393536E-928.7999155
GTCTCAC32250.028.3573781
GTATTAC5750.027.5516051
TTCACCG7700.027.1168065
GTATAAC4850.026.7253131
GTCTTAC9250.026.4684931
TTGCTCT31600.026.4303024
GTGTTAC10100.026.3798541
TCTGTCA32050.025.3852628
GTATAGC5200.024.9264951
CTTGCTC30350.024.6720873
GTACGAA1500.001555125124.0032921
Produced by FastQC (version 0.11.5)