FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352145
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15050.044.0068978
CTGTCGC19200.035.244889
GTCTCGC16750.034.2346421
TCGCTCT17150.034.0272874
GTCTTGC29450.034.0131871
GTATTAC6850.030.7298851
GGTATAC8250.029.0344451
TCTCGCT19700.027.085392
CTCGCTC19200.026.6573183
CGCTCTG19450.026.6490865
TAATACT9650.026.1304654
GTTTCAC50250.023.3835723
TTGCTCT40800.022.955654
GTATAGG10150.022.884291
GTACTAT8250.022.8756241
GTGTTAC15300.022.7721141
GTCTTAC14350.022.2563671
GTGTTAG24950.022.1103971
GTGCTAT13200.021.995791
GGGTTTC53250.021.67361
Produced by FastQC (version 0.11.5)