FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339461
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.052.110518
CTGTCGC16800.042.4213339
GTCTCGC14950.040.3189741
TCGCTCT15600.036.4798974
GTCTTGC29850.033.087741
TCTCGCT17500.030.4962312
CTCGCTC18450.028.9138183
GGTATAC6500.027.931821
CGCTCTG19950.026.7022135
GTCTCAC38900.025.5766431
TTACACT15500.024.1670024
GTGTTAG22750.023.941561
GTCTTAC13150.023.7473581
GTACTAA7150.023.361161
TTGCTCT40550.023.0942384
GGATAAT13300.022.9334951
TCACTCT43700.022.9131344
GTGTAAT14250.022.423861
GTTTCAC46700.022.0744193
CTTGCTC39850.021.346363
Produced by FastQC (version 0.11.5)