FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3339461
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13150.041.5365941
TCTGTCG14200.041.0653728
GTCTTGC29150.036.2263681
TCGCTCT15650.034.960564
CTGTCGC20350.030.423789
TCTCGCT18300.030.3504312
CGCTCTG17950.029.67885
CTCGCTC19950.028.5077743
TACGCCG1306.758809E-427.6889045
GGTATAC7100.026.6692241
GTCTCAC36950.026.4110551
TTGCTCT37950.026.17864
GTGTTAG22300.025.4732951
GTCTTAC12600.024.8538281
CTTGCTC40350.024.621513
GTGTTAT11400.024.2758331
TAATACT8700.023.997054
GTATTAG11900.023.8678341
GGGTTTC49000.023.037271
GTGTTAC12200.022.6839751
Produced by FastQC (version 0.11.5)