FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140361
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.048.861128
GTCTCGC15200.040.135991
TCGCTCT15350.038.023864
GTCTTGC25050.036.241031
CTGTCGC15900.033.5041859
TCTCGCT18200.030.9113672
CTCGCTC18150.030.5908363
CGCTCTG18600.028.6406755
GTCTCAC34950.028.2611641
TTACACT11450.027.0609174
GTATAAT5850.026.071241
TTGCTCT35700.025.432084
GTGTTAG19900.025.1822361
CTTGCTC35250.024.5470543
TACACTG17600.024.5416095
TCTGTCA36500.024.4564428
GTTTCAC37800.024.4171773
GTATAAC5150.023.9739691
CTATACT6950.023.8463844
GGTATAC6400.023.8307421
Produced by FastQC (version 0.11.5)