FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140361
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14000.043.70978
GTCTCGC13600.041.2165151
GTCTTGC28600.039.7081
TCGCTCT15450.037.277644
TCTCGCT17800.032.006742
CTGTCGC19100.030.9077519
CTCGCTC19150.029.3232943
CGCTCTG18700.029.258965
GGTATAC5750.027.8531511
TTGCTCT36450.027.848934
GGGTTTC40700.027.3663251
GTTTCAC41050.027.1835063
GTCTTAC10550.026.221081
GTATTAT6450.025.9589771
GTGTTAG18850.025.875151
TAATACT6750.025.5973134
TCTTGCT41400.025.0839822
GTCTCAC37700.024.909661
CTTGCTC38750.024.8954523
GTATAGC7950.024.723851
Produced by FastQC (version 0.11.5)