FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2894258
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC26350.033.956221
TCTGTCG11550.032.412038
GTCTCGC11600.030.1008011
CTGTCGC15750.025.5971939
GGTATAC6550.024.4329661
GTATTAG8900.023.7029721
GTATACG2151.8194678E-523.6839661
GTGTAAT10950.023.251381
TCGCTCT14300.023.1582984
TTGCTCT38650.021.9794794
GTTTCAC45000.021.9175973
GTGTTAC12050.021.732531
GCGTAGT3355.3158146E-821.714511
TCTCGCT14850.021.3642122
GTGTTAG20200.021.2468951
GTATTGG8800.020.6657981
GGGTTTC49750.020.6167811
GTATAGC5650.020.599961
GTGCTAC12450.020.450011
GTATAAC5800.020.0672021
Produced by FastQC (version 0.11.5)