FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943790
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12200.039.5344738
GTCTCGC13900.037.6146551
TCGCTCT14350.037.1461524
CTGTCGC15650.032.659169
CGCTCTG15450.031.6841015
TCTCGCT16700.029.7967432
GTATAAC6050.028.8068161
CTCGCTC17450.028.094683
GTATTAG10900.027.9809341
GTCTTGC27500.026.9343741
GTACTAC4650.026.548221
GTACTAT6650.026.2077051
GTATACG2258.9900277E-725.8194431
GTGTTAG18750.025.1739581
GGTATAC6550.024.3905071
TCCGTCG1500.001557925723.9960488
GTCTTAC11650.023.686291
GTATAGA9700.023.2075521
TAGTACT6600.022.9197224
GTGTAAC8650.022.6666361
Produced by FastQC (version 0.11.5)