FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2943790
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12600.037.70968
GTCTTGC26550.030.7097151
GTGTTAC12800.029.0056441
GTATTAG9350.028.8079531
GTCTCGC10850.026.8381481
CTGTCGC17750.026.7685039
CCGTTAC1801.4794442E-424.2661611
TAATACT10150.022.6966864
TCGCTCT13700.022.5957324
TATAGCG1600.00224590722.5335642
TTGCTCT37200.022.2552974
GTCTCAC33900.022.1187111
GTCTTAC11250.022.0013181
GTATTAC7350.021.790021
CTCGCTC14500.021.3490723
TCTCGCT15300.020.7367692
ATACTGA13550.020.7206736
GTGTATA18050.020.56911
GTTTCAC45500.020.5688723
TAGCGTC1750.003812876320.568879
Produced by FastQC (version 0.11.5)