FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881621
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12350.041.970018
CTGTCGC14650.037.3464669
GTCTTGC25800.035.7315441
GTCTCGC12800.030.0561731
GTCTTAC12450.029.7350461
GTATTAC5250.027.652761
TCGGTAC1358.346686E-426.7066043
TCGCTCT14450.026.4262684
GTATTAT8550.025.4696461
GTGTAAG14350.025.2921561
GTGTTAC11300.025.0526681
TCTCGAT10700.024.8938147
TCGATCT11250.024.3166989
CTCGATC11350.024.1024538
CGCTCTG17200.023.8572255
GGGTTTC48750.023.8239151
TCTCGCT15250.023.1751142
GTTTCAC43600.023.1538933
GTATAAG7650.022.772861
TTCACCG8600.022.6015825
Produced by FastQC (version 0.11.5)