FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897846
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.041.7595068
GTCTCGC12300.041.3133131
CTGTCGC14850.033.4491549
TCGCTCT14250.031.8500824
GTCTTGC27200.030.6920591
TCTCGCT15600.030.0485732
CTCGCTC16200.027.5925393
CGCTCTG16850.026.4883165
GTGTTAC13000.026.2453021
GTATTAT9600.025.710161
GTACTAC5050.024.437381
GGTATAC7250.024.0309161
GTCTCAC32950.023.3532621
GTATAAT10900.023.3098051
GTGTATA16000.023.1391431
GTCTAGC11700.022.9568841
GTATTAC7000.022.8150671
GGGTTTC49950.022.5264781
GTTTCAC47500.022.0084463
GTGTAAA19500.021.591881
Produced by FastQC (version 0.11.5)