FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897846
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12900.039.6239558
GTCTCGC12700.037.8141981
TCGCTCT14100.033.188184
CTGTCGC17500.031.676849
TCTCGCT15550.030.1428682
GTCTTGC26050.029.8875831
CTCGCTC15550.027.7785783
TTCACCG9000.027.1972875
CGCTCTG16150.026.300785
TCGTACT1658.835933E-526.1792054
GTATCGC1409.789551E-425.9870321
GTATACG1902.1412602E-422.9780081
GTCTCAC34900.022.9341141
GTGTTAG21200.022.6528471
GTATAGG7400.022.6157421
ACCGTGT10950.021.6964658
GGGTTTC51650.021.2726381
TTGCTCT37150.021.123064
GTTAGCC16100.021.0165353
GTATTAA9050.020.9044861
Produced by FastQC (version 0.11.5)