FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100081
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.041.043358
GTCTCGC13400.036.8441731
TCGCTCT14150.033.1010634
GTCTTGC28650.031.677441
CTGTCGC15650.028.0595079
GTATTAG8850.027.8933281
TCTCGCT17850.025.8632322
CGGATCG1400.00104208625.7102629
CGCTCTG18050.025.5250915
GTATAAC4900.025.1893841
CTCGCTC18900.025.1823833
TTGCTCT40200.024.915744
GTATAAG9150.023.8048171
TAATACT9050.023.0905594
GTGTTAC11950.023.0876871
GGCTAGA18050.022.1233141
GTGTTAA12700.021.724241
TACACTG19600.021.6700785
GTCTCAC35900.021.639841
GTGTATA14700.021.2381081
Produced by FastQC (version 0.11.5)