FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3100081
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15050.037.7253651
TCTGTCG13950.034.576938
TCGCTCT15350.029.0783084
GTCTTAC11950.028.6289391
CTGTCGC20050.028.3660329
TCTCGCT18400.027.8298992
GTCTTGC28300.027.2643261
CTCGCTC18750.024.957323
ACCGTGT10850.024.5503588
CGCTCTG17400.024.4111735
GTGCGGA6350.024.0724831
GTGTTAG20800.023.4469721
GTACTAG5750.022.7866231
CCGTGTT9900.022.5430349
TTACACT15650.022.540714
GTATAGG9150.022.2747211
TACACCG2603.40947E-622.1514685
GTGTTAC12550.022.0401821
GTCTACG2353.5995967E-521.6823061
GTGTATA19450.021.331951
Produced by FastQC (version 0.11.5)