FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607708
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.038.0369768
GTCTCGC12550.037.325881
TCGCTCT13250.034.2332764
CTCGCTC13900.032.1452833
GGCGTAC1652.502402E-630.5741581
GTCTTGC24300.029.0643221
CTGTCGC14000.028.7994279
TCTCGCT14600.028.6296162
GGTATAC4300.028.4918631
GTATAAT7350.027.4543441
CGCTCTG14900.026.5766495
GTACTAT5700.026.551241
GTGTTAG17400.026.0934581
TACACTG14950.025.0429785
GTGTTAC10950.025.0097791
GATCCGA1801.5945238E-423.9995236
GTATTAC6100.023.6287381
GTTTCAC40850.023.2874683
GTGTACG3102.5802365E-823.247631
GTATTAG7550.021.9543841
Produced by FastQC (version 0.11.5)