FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2607708
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10650.037.8591581
TCTGTCG10850.035.8341038
GTCTTGC23700.034.025321
TCGCTCT11550.033.0389634
GGTATAC4200.032.5714261
CTGTCGC14900.028.9932889
GTATTAG6700.027.9402981
TCTCGCT15100.027.6556282
CTCGCTC14350.026.0905913
GTCGTAC1400.001041090625.7142851
GTATAGG5650.025.4867271
GTATTAC5750.025.0434781
CCGTTCG1450.001277476224.8275859
GTATAGA7650.024.4705891
TAACACT11600.024.2068964
CGCTCTG15250.024.0786885
GTATAAA9200.023.478261
GTGCTAC9500.022.7368411
ACCCGTA1902.2997562E-422.7368417
GTATAAG7400.022.3783781
Produced by FastQC (version 0.11.5)