FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515185
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11100.042.1611338
CTGTCGC13500.036.7991039
GTCTCGC10700.030.9888611
GTCTTGC25200.029.4624081
GTATTAC6900.028.2063061
TCGCTCT13650.027.4279024
GTATAAC4250.027.1370491
GTACTAT5250.026.0871031
GTGTTAG16000.025.6794931
GTGTTAC9500.025.0392821
CTCGCTC14750.024.4348433
GTCTTAC10750.024.139351
GTATTAG9600.024.0275961
TCTCGCT13550.023.938932
GTGTAAC8550.023.6060561
CGCTCTG13950.023.2252395
TAGTACT5400.022.6661134
GTATTAA7450.022.2537441
GTCCTAC9950.021.7335031
TAATACT7650.021.6465324
Produced by FastQC (version 0.11.5)