FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2515185
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.036.488691
TCTGTCG10200.035.2941178
TCGCTCT12200.033.6393434
GTCTTGC22350.030.9261741
CTCGCTC13600.028.0588233
TCTCGCT14050.027.6725962
GTGTTAC10050.027.223881
GTACTAG5850.025.8461531
CTGTCGC14150.024.9328619
TTCACCG7300.024.6575365
GGTATAC5000.024.4800011
ACCGCGA1500.0015563724.08
CGCTCTG15400.023.8441565
TACCCCG2852.974266E-722.7368415
CTTGCTC30600.022.1176473
GTATTAG9500.021.9789471
GAGTGCA59300.021.8549776
GTATTAT7950.021.735851
TCACCGT8650.021.6416176
GGGTTTC46050.021.4201951
Produced by FastQC (version 0.11.5)