FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2742917
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11550.039.309211
TCGCTCT12800.036.561784
TCTGTCG10700.036.335738
TCTCGCT13200.031.1197932
GTATTAG10250.030.9360311
GTACTAT6700.030.1175061
GTCTTGC25650.030.062991
CTCGCTC14500.029.8207823
CGCTCTG14300.029.2022195
CTGTCGC14400.027.4994569
GTGTAAC10100.026.4007421
GTATTAT9250.025.710351
GTATTAA9100.025.3422031
GTGTAGT15050.024.4213371
TTATACT8100.023.9995254
TAATACT8200.023.706854
GTCTCAC32950.022.7464541
GTATAAT7650.022.6092191
GTGTAAT11900.022.4073521
GTATAAC4850.022.2887291
Produced by FastQC (version 0.11.5)