FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743669
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.043.2667668
CTGTCGC13650.033.2301259
TCGCTCT13250.030.9729844
GTCTCGC11500.030.7062071
GTCTTAC11350.029.2071971
GTCTTGC26250.029.100771
GTATTAC5850.027.1015851
TACACTG14650.027.0301935
CTCGCTC16100.025.0662923
TCTCGCT14700.025.0023462
GGGTTTC44800.024.7725451
GTATACG2051.3528912E-524.607761
TTACACT11150.024.5376424
CGCTCTG14650.024.0814445
GTATCGG1500.001548176724.0218621
GTCTCAC30700.023.9436131
GTATAAT7250.023.8561941
GTGTTAC10600.023.795241
GTATTAG8500.023.7392521
GTACCGT1851.9079463E-423.3726221
Produced by FastQC (version 0.11.5)