FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2743669
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11050.037.7918241
TCGCTCT12400.037.161264
GTCTTGC23700.035.5442731
TCTGTCG11850.034.0252888
GTGTAAT10500.033.5999681
TCTCGCT14350.030.6062432
CTCGCTC13950.030.4515843
GTGTTAG19000.029.178921
CGCTCTG13600.026.9999755
GTATTAA8550.026.105241
CTGTCGC16400.025.9024169
TTGCTCT33450.025.8295754
GTACTAT5050.025.6633431
GTTAGCC16300.024.2944583
GTATAAC4850.023.7525561
TACACTG17250.023.373895
TCTGTCA32450.023.297368
GTATTAG7450.023.194611
GTATAAT6550.023.0839481
TGTTAGC18800.022.9787032
Produced by FastQC (version 0.11.5)