FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977751
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12800.042.7488948
CTGTCGC13450.042.2888689
GTCTCGC12950.036.739681
TCGCTCT14650.035.384754
GTCTTGC26700.034.288911
TCTCGCT16400.029.8900242
CTCGCTC17500.028.8350853
CGTTTCG2003.4621917E-728.7992539
TTCACCG7550.026.7012965
CGCTCTG16650.026.3776955
TTGCTCT38100.025.700124
GTGTTAC11900.025.442721
GTCTTAC11700.025.2615031
GTATTGG9300.024.8043731
GTATTAT9150.024.4231591
CTTGCTC37300.023.3850213
TCACCGT8700.023.1718146
GGGTTTC48350.023.1098141
ACCGTGT9100.022.944468
GTGTATA15850.022.7406021
Produced by FastQC (version 0.11.5)