FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977751
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13500.044.266671
TCTGTCG11400.044.2105268
TCGCTCT15600.036.04
TCTCGCT16750.033.958212
GTCTTGC27150.032.8839761
CTCGCTC16850.031.192883
CGCTCTG16050.030.5046755
CTGTCGC17550.030.3589749
GGTATAC6550.027.4809171
GTCTTAC11550.025.5584411
GTCTCAC36000.024.21
GTGTAAT12650.023.9051381
GTATTAG9650.023.8756471
TTGCTCT38800.023.7525774
GTATTAT9450.023.6190471
CTTGCTC37450.023.2630183
GTGTAAG14100.022.9787241
TAATACT7550.022.8874174
TTCACCG8500.022.8705885
GGGTTTC48700.022.6201231
Produced by FastQC (version 0.11.5)