FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820652
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11650.040.788848
TATACCG1252.8736213E-740.319155
GTCTCGC12800.039.9770551
TCGCTCT12250.038.2032784
CTGTCGC15250.034.936979
TCTCGCT14850.033.9730072
CTCGCTC14850.032.517023
GTATTAC7350.030.3974191
GTCTTGC26200.028.8835371
GTATTAT8550.028.6599351
GTGTTAC10300.027.9888571
CGCTCTG15300.027.764125
GTATACG2156.457158E-726.817231
CTTGTCG2706.000846E-926.6661059
GTATTAG8400.025.7397541
GTATTAA8800.025.3887561
TACACCG2351.4148663E-624.5101225
GGTATAC6300.024.0237671
GTATAAT9050.023.891041
GTGTTAG19250.022.838181
Produced by FastQC (version 0.11.5)