FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820652
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.046.9564868
CTGTCGC18600.034.4515849
GTCTCGC13200.034.3636051
GTCTTGC25700.033.89881
TCGCTCT15500.031.587074
TAATACG2253.0171577E-828.7999744
CGCTCTG16200.028.444425
GTACGGT2405.6452336E-826.9999751
TCTCGCT17450.025.5816422
GTGTTAG19250.025.059721
GTATTAG9550.024.879561
GGGTACG2651.4735451E-724.452811
TTATACT8550.024.421034
CTCGCTC18750.024.1919783
GTCTTAC13200.023.9999791
TAATACT8850.022.7796424
GGAGTGC61950.022.779645
TTGCTCT36450.022.3209694
GTATTAC6850.022.0729751
GTTAGCC15100.021.9337563
Produced by FastQC (version 0.11.5)