FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2851558
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.045.9452978
CGTACTA1054.0131654E-641.142665
CTGTCGC19200.033.3748369
GTCTCGC11700.032.6152271
TCGCTCT13100.031.328094
GTCTTGC24000.028.1998631
CGCTCTG15050.027.7473745
GTATTAA8350.027.5926821
GTCTTAC11750.027.5743331
GTATTAT8900.026.6964991
GTGTAAG14400.025.4998761
CTCGCTC16700.025.0058673
GGTATAC5500.024.8726041
GTATATA11700.024.6152651
TCTCGCT15550.024.5400732
GTATTAG8950.023.3294941
GTACTAC5050.022.8117711
GTGTTAC11850.022.4809041
TTGCTCT37550.022.0504934
TATACTG7550.021.9336685
Produced by FastQC (version 0.11.5)