FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3078267
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11850.034.6313868
GTCTCGC13800.032.383751
TCTCGCT16250.026.1654622
TCGCTCT15550.025.9272774
CTCGCTC16400.025.4867153
GTCTTGC30200.024.3448851
CTGTCGC17250.024.207639
CGCTCTG17700.023.1850415
GTATAAG10650.023.011441
GTACAGA15750.022.8825241
GTGTTAG24300.022.8401491
GGTATAC6700.022.5922241
GTCTTAC12200.022.4511341
GTACTAG6450.022.3503721
GTGTAGA17300.022.08231
GTATTAA10850.021.9229351
GTACTAC6250.021.9123061
GTCTAAG11850.021.8977071
GTATTAT12400.021.5077291
GTCTCAC36450.021.1592731
Produced by FastQC (version 0.11.5)