FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2140903
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9450.035.8129161
TCTGTCG9650.035.0665868
TCGCTCT9800.033.0604974
GTACTAA4750.028.802731
GTCTTGC18950.028.118761
CGCTCTG11700.026.4609555
GTGTTAA8450.026.4167041
CTCGCTC12000.025.799433
GTCCTAC6850.025.2286661
GGGTAAC7000.024.6880511
CTGTCGC15700.023.8466079
TCTCGCT12500.023.6154792
GTGTTAG14150.023.4085791
GTCTCAC27400.022.863481
GTATTAC5450.022.4608441
GTGTAAT9300.022.453741
GTACAGA12600.022.2878251
TCTGTCA28450.022.0170888
TTACACT10150.021.9896624
GTCTTAC8000.021.6020451
Produced by FastQC (version 0.11.5)