FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734726
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.035.8182458
GTCTTGC27000.034.4476361
GTCTCGC12150.033.824551
TCGCTCT13100.031.8723964
CTCGCTC15350.026.7672983
GTATAAT8650.026.672721
TCTCGCT15150.026.644862
GTGTTAG18450.026.1825471
CTGTCGC14300.026.1773269
GTGTAAC11150.025.8653621
GTATTAC7250.025.8564471
GTATACG2001.1131324E-525.2348941
GTATTAG10700.024.9316711
GTCTTAC13450.024.6586321
CGCTCTG15500.024.6151315
GGAGTGC63550.023.6749325
GTATTAA9950.023.1878431
GTGTATA16200.022.6980531
GTATAAG11300.022.3317661
TTGCTCT39050.022.3060324
Produced by FastQC (version 0.11.5)