FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734726
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC27150.034.479191
TCTGTCG12800.033.7490358
GTCTCGC11300.032.4993971
GTATTAG10400.029.772731
TCGCTCT14000.028.7991734
CTGTCGC17350.026.5583159
GTATAGA9350.024.6446081
TCTCGCT15650.023.92222
GTCTTAC11650.023.487741
TTGCTCT39800.023.3360144
GTTTCAC47350.023.264383
GTGTAAC8700.023.1751381
GGGTTTC51600.023.1655141
GTGTAAG14250.022.7395151
CTCGCTC16200.022.2215843
GTATTAC9250.021.7971571
GTGTTAC11250.021.7625581
GTGTAAT13600.021.7084351
TACGCCA2003.254253E-421.599385
CTTGCTC37400.021.5608793
Produced by FastQC (version 0.11.5)