FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966835
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14400.036.0037571
GTCTTGC29200.032.304741
TCGCTCT16650.030.7028054
TAGTCCG950.00489149130.3158915
TCTGTCG14300.028.1958968
CTCGCTC18600.026.7097663
TCTCGCT19300.026.4866872
CGCTCTG17100.025.6842965
GTATTAA8750.025.5112341
GTGTTAC13800.024.5243021
TATACTG9650.023.8757275
GTATTAG11500.021.9153331
TTGCTCT41200.021.844734
GTGTTAG23550.021.403511
TAATACT10450.021.3589214
GTATATA14900.021.2639661
GTATAGA9950.020.9871161
GTATAGT8300.020.8214511
GTACAGA16950.020.8163321
GTGTAAA20500.020.3728581
Produced by FastQC (version 0.11.5)