FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2996953
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14100.040.848348
GTCTCGC13650.037.4955941
TCGCTCT14450.035.37484
CTGTCGC19000.031.4505339
CTCGCTC17500.030.477243
TCTCGCT17450.030.1520392
GTCTTGC31200.030.0360811
CGCTCTG16900.026.4124375
GTGTTAC14050.026.1666661
GTATTAC8000.023.4281461
GTATTAG13400.023.1322671
GTCTCAC34050.022.8644711
GTCTTAC14850.022.8152891
GTATAAC6650.022.7641891
TTGCTCT43050.022.576894
GCGTAAC1600.002249685322.5270611
GTGTAAT15100.022.437551
GTATTAA11600.022.3717021
GTGTTAA14700.022.0673271
GTATTGG11850.021.8997271
Produced by FastQC (version 0.11.5)