FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916896
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.034.0917248
GTCTTGC29350.033.363861
GTCTCGC12300.030.4399321
TATACCG1306.753923E-427.6921845
TCGCTCT15050.027.2689844
GTATTAG10300.025.16581
GTCTTAC10850.024.5537281
GTCTCAC34500.024.4181211
CTTGCTC37050.023.7083993
GTGTAAA20350.023.7058681
TCTCGCT16350.022.4582332
TTGCTCT40750.022.4391654
TCTGTCA38550.022.4123548
GTATAGA10350.022.2615361
GGTATAC6550.021.9853881
GAGTGCA73500.021.9427596
GTATATA12500.021.8886551
GTATACG1650.002708236221.8188341
GTGTAAC10000.021.6006451
CTCGCTC16050.021.5326163
Produced by FastQC (version 0.11.5)