FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201141
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8450.050.2681858
GTCTCGC9900.041.508761
GTCTTGC23400.034.1985131
TCGCTCT11050.033.2281234
CGTTTCG1153.2958767E-431.3018539
TCTCGCT12150.030.253492
CTGTCGC14050.030.2324689
CGCTCTG12050.029.2756775
TTGCTCT27750.028.5382654
GTCTCAC25850.028.168321
CTCGCTC13150.025.7604643
CTGTTGC23750.025.1601039
GTTTCAC32500.024.6162093
GTATTGC6450.024.5902581
TCGATCT8200.024.5834089
TCTCGAT8500.024.5627487
GGGTTTC37250.023.9991321
CTTGCTC27850.023.8091663
TCTGTCA29500.023.6726918
GTGTAAA12350.023.3503361
Produced by FastQC (version 0.11.5)