FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201141
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9550.039.2052541
TCTGTCG9650.037.306718
GTCTTGC20150.035.0183221
TCGCTCT12300.028.6837044
TTGCTCT28500.027.5375884
CTCGCTC13450.027.3018553
GTCTTAC8450.026.4149171
TCTCGCT13800.026.086482
TAATACT7250.025.821394
CTGTCGC16200.025.334029
GGAGTGC52800.025.2279575
GAGTGCA56600.024.8063266
GTGTTAC10500.024.6863861
GGGTTTC35700.024.4040221
AGGACCG3301.9226718E-924.000655
TACACTG14000.023.6577845
CGCTCTG13100.023.6342285
GTGTAAC6500.023.262171
CTTGCTC28250.023.193553
GTGTACG2802.5065674E-723.1434841
Produced by FastQC (version 0.11.5)