FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320363
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.042.662298
GTCTCGC10600.038.083071
TCGCTCT12000.035.9963074
CTGTCGC13450.034.2567069
TCTCGCT12400.033.1304972
CTCGCTC12400.033.1304973
CGCTCTG12950.031.6878665
GTCTTGC20800.031.537541
GTCTCAC26950.028.887821
GTATTAA7750.026.0439051
GGTATAC5150.023.7953131
GGGTTTC37300.023.5776631
TCTGTCA29700.022.785548
GTCCTAC7650.022.6151541
TCACTCT35150.022.5296924
GGCTAGA13500.022.4266971
GTTTCAC35250.022.2865163
GTATTAC5500.022.2810691
GTATTGC6800.022.2617931
TTGCTCT30900.022.1336524
Produced by FastQC (version 0.11.5)